Table 3.
AFLP and MSAP adaptors and primers used in this study and fragments obtained
| Sequences | Number of fragments | |
|---|---|---|
| AFLP oligonucleotides | ||
| Adaptors | ||
| EcoRI | 5′-AATTGGTACGCAGTCTAC | |
| 5′-CTCGTAGACTGCGTACC | ||
| MseI | 5′-TACTCAGGACTCAT | |
| 5′-GACGATGAGTCCTGAG | ||
| Preselective primers | ||
| EcoRI + A | 5′-AGACTGCGTACCAATTCA | |
| MseI + C | 5′-GACGATGAGTCCTGAGTAAC | |
| Selective primer combinations (EcoRI+3 and MseI+3) | ||
| EcoRI + ACG/MseI + CTG | 137 | |
| EcoRI + ACG/MseI + CTC | 64 | |
| EcoRI + ACA/MseI + CTC | 197 | |
| EcoRI + AAG/MseI + CAC | 191 | |
| EcoRI + ACA/MseI + CTG | 73 | |
| EcoRI + AAG/MseI + CTG | 170 | |
| Total: | 832 | |
| MSAP oligonucleotides | ||
| Adaptors | ||
| EcoRI | 5′-CTCGTAGACTGCGTACC | |
| 5′-AATTGGTACGCAGTCTAC | ||
| HpaII – MspI | 5′-GACGATGAGTCTCGAT | |
| 5′-CGATCGAGACTCATC | ||
| Preselective primers | ||
| EcoRI + 0 | 5′-GACTGCGTACCAATTC | |
| HpaII – MspI + A | 5′-ATGAGTCTCGATCGGA | |
| Selective primer combinations (EcoRI+3 and HpaII – MspII+3) | ||
| EcoRI + AGA/HpaII – MspI + ATC | 46 | |
| EcoRI + ACA/HpaII – MspI + AAT | 46 | |
| EcoRI + ACA/HpaII – MspI + ATC | 43 | |
| EcoRI + AAC/HpaII – MspI + ATC | 40 | |
| EcoRI + AAC/HpaII – MspI + AAT | 57 | |
| Total: | 232 | |