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Chromosome banding (A, C, E, I), in situ hybridization (B, D, F, J), and GISH (G, H, K, L) for (A and B) Epidendrum fulgens, (C and D) E. puniceoluteum, (E–H) hybrid/2n = 38 and (I–L) hybrid/2n = 40. Arrows in (A, C, E, and I) indicate DAPI+ bands (blue) and asterisks indicate CMA+ bands (yellow). Arrowheads in (B, D, F, and J) indicate 5S rDNA (red) and asterisks show 45S rDNA (green). Scale bar in (K) is 10 μm.
