Figure 1.
Kinetic studies of CM between SBL-156Seac (6a) and allyl alcohol. (a) LC-MS of reaction showing conversions over time. (b) Changes in [product] as a function of time. Inset shows kinetics of CM with SBL-156Sac (9a); note difference in time scale. Conditions: [protein] = 0.01 mM, [cat.] = 1 mM, [allyl alcohol] = 52 mM, [MgCl2·6H2O] = 26 mM in 3:7 tBuOH/NaPi (50 mM, pH 5) at room temperature. (c) CM of 6a with various concentrations of allyl alcohol. (d) Determination of the second-order CM rate constant for 6a.