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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: J Neuropathol Exp Neurol. 2013 Oct;72(10):10.1097/NEN.0b013e3182a5f96e. doi: 10.1097/NEN.0b013e3182a5f96e

Figure 4.

Figure 4

Compromised neuromuscular junction (NMJ) integrity in the soleus muscle of Trembler J (TrJ) mice. (A, B) Longitudinal sections (20 μm thick) from wild type (Wt) (A) and TrJ (B-B”) soleus muscles were reacted with anti-phosphorylated neurofilament-heavy chain (pNFH) antibody (green) and α-bungarotoxin (α-BTX, red) to stain post-synaptic acetylcholine receptors. Samples from Wt mice (A) have thick, well-defined, branched axon terminals in close association with the post-synaptic muscle cell. NMJs from TrJ mice (B-B”) show features of altered innervation, i.e. terminal sprouting (B, *), thinning (B’, arrows) and axon retraction (B’’, arrowheads). Scale bar: 10 μm. (C) Percentages of intact NMJs were quantified in sections of soleus muscles from Wt and TrJ mice at the indicated ages. At 10 months of age, TrJ mice have a significant reduction compared to both 2 and 5 months (Student t-test, **p < 0.01, ***p < 0.001, #p < 0.01). n = 5 mice per age and genotype.