Lungs and lung-draining lymph nodes (LDLNs) were harvested 8 days post-RSV infection, and tissues from Beclin-1+/− mice were compared to those from WT mice using flow cytometry. (A) Representative flow plots of CD11b+ and CD103+ DCs from collagenase-digested lungs of Beclin-1+/− or WT littermate mice. Cells were gated on CD11c+ MHC-IIhigh according to the scheme in Suppl. Fig. S1. Total numbers of CD11b+ and CD103+ DCs (B) were calculated using percentages and total cell counts within lungs. (C) Representative flow plots of CD11b+ and CD103+ DCs in single-cell preparations of LDLNs of Beclin-1+/− or WT littermate mice. Total numbers of CD11b+ and CD103+ DCs (D) were calculated using percentages and total cell counts within LDLNs. (E) Total CD45+ cell counts from lungs, (F) LDLNs, and (G) lung inflammatory CD11b+ DCs co-staining with FcεRIα+ (clone MAR-1) and Ly6C+ were calculated by flow cytometry. (H) Median fluorescence intensity (MFI) of MHC-II surface staining on CD11b+ and CD103+ DCs from LDLNs. Data are representative of at least three independent experiments, with four to six mice per group. Error bars represent SEM. *p<0.05, **p<0.01, ***p<0.001.