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Flow chart of affinity method to isolate 26S proteasomes from mammalian cells and tissues. GST-UBL, recombinant fusion protein of glutathione S-transferase (GST) and the ubiquitin-like domain (UBL) of human HR23B; His10-UIM, recombinant protein containing the second ubiquitin-interacting motif of human S5a; GSH-Sepharose, glutathione-coupled resin with affinity for GST; Ni-NTA, nickel-charged nitrilotriacetic acid (NTA) agarose that binds to His tags.
