Table 1.
Proteasome-Associated Proteinsa
| unigene | protein | peptides | methods |
|---|---|---|---|
| Proteasome Activators | |||
| Rn.163607 | PA200 | 37 | GG |
| Rn.2742 | PA28α | 14 | GG |
| Rn.198325 | PA28γ | 3 | GG |
| Proteasome-Associated Proteins | |||
| Rn.103325 | PI31 | 14 | GG |
| Rn.154631 | Ecm29 | 164 | GG |
| Rn.9320 | Rpn13 | 10 | GG |
| Rn.40424 | Uch37 | 51 | NP/GG |
| Rn.11790 | Usp14 | 32 | NP/GG |
| Rn.198497 | E6-AP/E3A | 20 | GG |
| UBL-UBA Proteins | |||
| Rn.107103 | Sequestosome/p62 | 6 | GG |
| E3 Ligases | |||
| Rn.37755 | p600/UBR4 | 159 | NP/GG |
| Rn.12130 | HUWE1/ARF-BP | 116 | NP/GG |
| IPI00196914 | HECT E3b | 7 | GG |
| p97 and N-Terminal Adaptors | |||
| Rn.98891 | p97 | 83 | GG |
| Rn.144645 | NPL4 | 6 | GG |
| Rn.11946 | UFD1 | 8 | NP/GG |
| Rn.2771 | p47 | 14 | GG |
| Rn.198539 | SAKS1 | 6 | NP/GG |
| Deubiquitinating Enzymes | |||
| Rn.44078 | USP5 | 138 | NP/GG |
| Rn.72721 | USP7 | 17 | GG |
| Ubiquitin | |||
| Rn.106034 | ubiquitin | 18 | NP/GG |
| Transcription | |||
| Rn.33229 | STAT1α | 76 | GG |
| Translation | |||
| Rn.144660 | |||
| Rn.965 | EF1α | 7 | NP/GG |
| Rn.55145 | EF2 | 3 | NP |
| Chaperones | |||
| IPI00358052 | putative, Hsp40/DnaJ familyc | 5 | GG |
| Rn.120392 | |||
| Rn.201298 | Hspa8, Hsp70 family | 5 | GG |
| Rn.11088 | Hspa5, Hsp70 family | 2 | NP |
| Rn. 1950 | Hspa1, Hsp70 family | 2 | GG |
| Cytoskelton and Myofibrillar Proteins | |||
| Rn.82732 | actin, alpha 1 | 59 | NP/GG |
| Rn.94978 | actin, beta | 5 | GG |
| Rn.101923 | dynactin 2 | 4 | NP |
| Rn.84920 | myosin, light chain 1 | 17 | GG |
| Rn.87540 | tropomyosin 1, alpha | 7 | GG |
| Rn.17580 | tropomyosin 2 | 9 | GG |
| Rn.43529 | troponin C2 | 2 | GG |
| Rn.15488 | troponin T3 | 10 | GG |
| Rn.2710 | vimentin | 2 | GG |
| Metabolism | |||
| Rn.129558 | glyceraldehyde 3-phosphate dehydrogenase | 13 | NP/GG |
| Rn.122663 | aldolase A-like | 8 | GG |
| Rn.1774 | aldolase | 7 | GG |
| Rn.1556 | pyruvate kinase | 4 | NP |
| IPI00388191 | enolasec | 2 | NP |
| Rn.144555 | |||
| Rn.37838 | triosephosphate isomerase | 2 | GG |
| Rn.10756 | creatine kinase | 2 | GG |
| Rn.3001 | catalase | 8 | NP |
| Rn.9857 | arginase 1 | 4 | NP |
| Rn.10039 | transglutaminase 1 | 2 | NP |
| Rn.53317 | transglutaminase 3 | 2 | NP |
| Rn.9486 | fatty acid synthase | 2 | NP |
| Miscellaneous | |||
| Rn.22682 | similar to thioredoxin family Trp26 | 27 | GG |
| Rn.1792 | annexin A1 | 8 | NP |
| Rn.90546 | annexin A2 | 16 | NP/GG |
| Rn.141102 | premature ovarian failure 1B | 5 | NP/GG |
| Rn.203179 | glioblastoma amplified sequence | 7 | GG |
| Rn.92965 | ATP synthase | 2 | NP |
| Rn.218029 | ATPase, Ca2+ transporting | 2 | GG |
| Rn.91512 | glial fibrillary acidic protein | 6 | NP/GG |
| IPI00231340 | histone foldc | 3 | NP |
| Rn.11207 | thrombospondin 4 | 2 | NP |
| Rn.2845 | peroxiredoxin 1 | 2 | NP |
| Rn.2511 | peroxiredoxin 2 | 2 | NP |
| Rn.68633 | tubulin tyrosine ligase-like family | 2 | NP |
| Rn.24735 | nuclear RNA export factor 7 | 2 | NP |
| Rn.172813 | Mab-21-like 2 | 2 | GG |
| Rn.161841 | |||
| Rn.2202 | cytochrome c | 2 | GG |
| IPI00367223 | similar to CG1193-PAc | 2 | GG |
a
Sum of unique peptides identified by indicated methods: NP (proteins identified in bands of doubly and singly capped proteasomes after native PAGE); GG (proteins identified in 26S peak of glycerol gradient). Databases: IPI database (http://www.ebi.ac.uk/IPI/IPIhelp.html); UniGene (http://www.ncbi.nlm.nih.gov/UniGene). Although cytoskeleton proteins are often found as contaminants or unspecifically interacting proteins in the study of protein complexes using mass spectrometry, identified candidates from this protein family are listed as hypothetical proteasome interacting proteins.
b
Not annotated in UniGene, termed HUWE in IPI database, but has no sequence similarity with HUWE1/Rn.12130.
c
Not annotated in UniGene.