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Sequence A is the 14 bp binding site for tetraintercalators 1 – 4. Sequences B and C are the 22 bp designed binding sites for hexaintercalator 5. Lines between base pairs indicate expected intercalation sites. Sequence C differs from sequence B by 2 bp, highlighted in the boxes, and also contains sequence A as underlined. Oligos A – C are the oligonucleotides used for gel-shift assays and UVvisible spectroscopy experiments.
