Table 1.
List of urinary ions exhibiting differential presence between genotypes and/or ethanol feeding.
| Comparison | Marker | m/z | RT (min)* | P value** | Fold change*** | Confirmed ID**** |
|---|---|---|---|---|---|---|
| Wild-type vs. Ppar!/! -null controls | N2 | 105.0187 | 0.3197 | 0.0357 | 61.6 | L-Glyceric acid |
| Wild-type vs. Ppar!/! -null controls | N4 | 145.049 | 1.4471 | 0.0095 | 40.01 | 2-Methylglutaric acid, Adipic acid |
| Wild-type vs. Ppar!/! -null controls | N1 | 431.183 | 6.3109 | 0.024 | 3.6 | |
| Wild-type vs. Ppar!/! -null controls | N3 | 553.3382 | 5.5091 | 0.0095 | 3.2 | |
| Wild-type vs. Ppar!/! -null controls | N9 | 161.0438 | 0.5371 | 0.0095 | 2.72 | |
| Wild-type vs. Ppar!/! -null controls | P2 | 281.0999 | 2.1687 | 0.016 | 0.57 | |
| Wild-type vs. Ppar!/! -null controls | N7 | 144.0654 | 0.8706 | 0.016 | 0.36 | Isobutyrylglycine |
| Wild-type vs. Ppar!/! -null controls | N8 | 128.034 | 1.0348 | 0.016 | 0.34 | Pyroglutamic acid |
| Wild-type vs. Ppar!/! -null controls | P1 | 172.0968 | 2.4299 | 0.036 | 0.28 | |
| Wild-type vs. Ppar!/! -null controls | N5 | 172.0963 | 3.1093 | 0.024 | 0.16 | Hexanoylglycine |
| Wild-type vs. Ppar!/! -null controls | N6 | 216.1231 | 3.7551 | 0.0095 | 0.13 | Propionylcarnitine |
| Wild-type controls vs. ethanol-fed wild-type | N11 | 129.0182 | 0.4204 | 0.03 | 2.22 | Glutaconic acid |
| Wild-type controls vs. ethanol-fed wild-type | P4 | 160.096 | 1.6379 | 0.016 | 1.6 | |
| Wild-type controls vs. ethanol-fed wild-type | P5 | 206.0452 | 1.6903 | 0.015 | 0.47 | Xanthurenic acid |
| Wild-type controls vs. ethanol-fed wild-type | N12 | 145.0129 | 0.3431 | 0.0043 | 0.36 | |
| Wild-type controls vs. ethanol-fed wild-type | N10 | 186.0758 | 1.5359 | 0.0043 | 0.25 | |
| Ppar!/! -null controls vs. ethanol-fed Ppar!/! -null | P6 | 211.0708 | 0.3344 | 0.036 | 2.96 | N-Acetylglutamine |
| Ppar!/! -null controls vs. ethanol-fed Ppar!/! -null | N14 | 87.0084 | 0.3171 | 0.036 | 0.6 | Pyruvic acid |
| Ppar!/! -null controls vs. ethanol-fed Ppar!/! -null | N13 | 175.0597 | 1.92 | 0.1016 | 0.52 | |
| Ppar!/! -null controls vs. ethanol-fed Ppar!/! -null | N15 | 161.0462 | 0.9361 | 0.036 | 0.31 | |
| Ppar!/! -null controls vs. ethanol-fed Ppar!/! -null | N16 | 124.0061 | 0.2852 | 0.36 | 0.3 | Taurine |
*
RT = retention time in minutes (min)l.
**
P-values as compared to respective control.
***
Fold change = (Average normalized intensity of second listed group)/(Average normalized intensity of the first listed group). If the fold change for a particular metabolite is less than unity it means that the average intensity value of that metabolites in first group was higher than in second group. These metabolites are specific to the comparison between each genotype/treatment comparison, and do not depict the same changes in their urinary excretion levels made in other comparisons.
****
Confirmed by MS/MS as described in Materials and methods.