Figure 1.
Read accuracy (ABIP) versus quality value (mean QV). The mean QV is correlated with the CCS read accuracy, particularly, at the range of QV-40 and above. It suggests that QV can be a useful QC parameter to remove low-quality CCS reads. However, the plot also shows that the majority of CCS reads, including the low-quality CCS reads, are below QV-40. Thus, a QV-40 cutoff might have a higher tradeoff by removing a large amount of high-quality CCS reads. A QV-30 cutoff may be more balanced.