Table 1.
A Comparison of read accuracy (ABIP) improvements across three quality control (QC) strategies and their impacts on the De Novo assembly results.
QC method | None | 50-bp trimmed at both ends |
QV-Based | spike-in trained SVR |
||
---|---|---|---|---|---|---|
# of CCS reads selected | all 9812 | all 9812 | top 3000 | top 5000 | top 3000 | top 5000 |
90% percentile of read accuracy | 99.44% | 99.48% | 99.62% | 99.56% | 99.62% | 99.56% |
50% percentile of read accuracy | 97.48% | 97.63% | 99.12% | 98.61% | 99.12% | 98.67% |
10% percentile of read accuracy | 92.98% | 93.06% | 98.44% | 94.56% | 98.54% | 95.09% |
De Novo Assembly: # of Contigs | 13 (3 FP*) | 10* (0 FP) | 11 (1 FP) | 12 (2 FP) | 10 (0 FP) | 10 (0 FP) |
Note*: final assembled length is 100 bp shorter.
FP denotes False Positive