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. Author manuscript; available in PMC: 2014 Apr 2.
Published in final edited form as: FEBS Lett. 2013 Feb 19;587(7):10.1016/j.febslet.2013.02.022. doi: 10.1016/j.febslet.2013.02.022

Figure 1.

Figure 1

Supercomplexes, COX and Cox1p intermediates in different COX mutants. Mitochondria of the parental strain W303-1A expressing untagged Cox1p, of MRS/COX1-HAC and of cox4, cox14, coa3 and coa1 mutants, each harbouring the COX1-HAC fusion gene were extracted with digitonin and fractionated as described in Materials and Methods. The fraction eluted from the protein C antibody beads with EDTA was separated by BN-PAGE on 4–13% polyacrylamide gel, transferred to a PVDF membrane and probed with a monoclonal antibody against the HA tag followed with peroxidase coupled anti-mouse antibody. Cox1p-HAC was detected with the Super Signal chemiluminescent substrate kit (Pierce, Rockford, IL).