Fig 2.
Expression analysis of R. leguminosarum dmeRF genes as a function of nickel or cobalt concentration in the culture medium. R. leguminosarum wild-type SPF25 (A and B) and dmeRF mutant D15 (C and D) containing reporter fusion plasmids pDL10 (dmeRF′-lacZ) (diamonds), pDL13 (dmeR′-lacZ) (squares), or pDL43 (dmeF′-lacZ) (triangles) were grown in TY medium supplemented with the indicated amounts of NiCl2 (A and C) and CoCl2 (B and D). β-Galactosidase activities are expressed in Miller units. Values are averages of three independent experiments. Error bars indicate standard errors.