Table 3.
Symbiotic expression of R. leguminosarum dmeRF genes
| lacZ fusion | β-Galactosidase activity (Miller units)a |
|||||
|---|---|---|---|---|---|---|
| SPF25 |
D15 |
|||||
| Control | Ni(II) | Co(II) | Control | Ni(II) | Co(II) | |
| pDL43 | 33 ± 12 | 39 ± 11 | 33 ± 6 | 33 ± 5 | 43 ± 15 | 46 ± 13 |
| pDL10 | 37 ± 12 | 35 ± 8 | 46 ± 7 | 40 ± 17 | 41 ± 13 | 113 ± 24 |
| pDL13 | 56 ± 17 | 60 ± 17 | 63 ± 17 | 333 ± 114 | 367 ± 141 | 367 ± 74 |
a
Values are β-galactosidase activities of pea bacteroids obtained from plants inoculated with R. leguminosarum SPF25 (wild type) and D15 (dmeRF deletion mutant) harboring dmeF′-lacZ (pDL43), dmeRF′-lacZ (pDL10), or dmeR′-lacZ (pDL13) fusions. Plants were grown in standard nutrient solutions (control) or in nutrient solutions supplemented with Ni(II) (85 μM NiCl2) or Co(II) (42.5 μM CoCl2). Values are the averages of four replicates ± standard errors.