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PNA-FISH outcome for ground beef samples artificially inoculated with 10 CFU/25 g of E. coli O157:H7 CCC-05-12. Results were obtained using a direct hybridization protocol without any additional sample pretreatment (A) or using a pretreatment with 1% Triton X-100 (B). It is possible to observe a decrease in the autofluorescence intensity for panel B in both red (I) and green (II) channels.
