Table 1.

Results of the EcoPNA1169 probe specificity and sensitivity test^c

Straina	Serotype	Isolation origin	Verotoxin production	PNA FISH outcome
E. coli CECT 4267	O157:H7	Human stool from outbreak of hemorrhagic colitis	Stx1, Stx2	+
E. coli CECT 4782	O157:H7	Human stool from outbreak of hemorrhagic colitis	Stx1, Stx2	+
E. coli CECT 4783	O157:H7	Raw hamburger meat implicated in hemorrhagic colitis outbreak	Stx1, Stx2	+
E. coli CECT 5947	O157:H7		stx2 gene has been replaced	+
E. coli NCTC 12900	O157:H7		NT	+
E. coli CCC-1-12	O157:H7	Fecal swab	Stx2	+
E. coli CCC-5-12	O157:H7	Fecal swab	Stx2	+
E. coli CCC-7-12	O157:H7	Fecal swab	Stx2	+
E. coli CCC-10-12	O157:H7	Fecal swab	Stx2	+
E. coli CCC-11-12	O157:H7	Milk filter	Stx2	+
E. coli CCC-12-12	O157:H7	Milk filter	Stx2	+
E. coli CCC-13-12	O157:H7	Milk filter	Stx2	+
E. coli CCC-14-12	O157:H7	Bovine milk filter	Stx2	+
E. coli CCC-15-12	O157:H7	Bovine milk filter	Stx2	+
E. coli CCC-16-12	O157:H7	Caprine milk filter	Stx2	+
E. coli CCC-18-12	O157b	Bovine milk filter	Stx2	+
E. coli CCC-23-12	O157:H7	Milk filter	NT	+
E. coli CCC-24-12	O157:H7	Milk filter	NT	+
E. coli CCC-25-12	O157:H7	Milk filter	NT	+
E. coli CCC-26-12	O157b	Milk filter	NT	+
E. coli CECT 352	O127a:K63(B8):H-		EPEC	−
E. coli CECT 504	O141:K85(B):H4	Swine edema	ND	−
E. coli CECT 515T	O1:K1(L1):H7	Human urine–cystitis	ND	−
E. coli CECT 533	O103:K-:H-		ND	−
E. coli CECT 727	O111:K58(B4):H-	Infantile gastroenteritis	EPEC	−
E. coli CECT 730	O55:K59(B5):H-		ND	−
E. coli CECT 736	O28a,28c:K73(B18):H-	Feces	ND	−
E. coli CECT 740	O125a,125b:K70(B15):H19	Gastroenteritis	ND	−
E. coli CECT 744	O158:K-:h23	Feces of infant with diarrhea	ND	−
E. coli CECT 832	O111:K58(B4):H-	Infantile gastroenteritis	ND	−
E. coli CECT 4537	O10:K5(L5):H4	Human peritonitis	ND	−
E. coli CECT 4555	O97:K-:H-		ND	−
E. coli CCC-2-12	O103	Fecal swab	NT	−
E. coli CCC-3-12	O26	Fecal swab	Stx1, Stx2	−
E. coli CCC-4-12	O26	Fecal swab	NT	−
E. coli CCC-8-12	O26	Fecal swab	NT	−
E. coli CCC-9-12	O26	Fecal swab	NT	−
E. coli CCC-19-12	O26	Caprine milk filter	Stx1	−
E. coli CCC-20-12	O26	Caprine milk filter	Stx1	−
E. coli CCC-21-12	O26	Bovine milk filter	Stx1	−
E. coli CCC-22-12	O26	Bovine milk filter	Stx1	−
E. coli CECT 434	O6	Clinical isolate	ND	−
E. coli N9	ND	Porcine feces	ND	−
E. coli N5	ND	Bovine feces	ND	−
E. coli ATCC 29425 (K12)	OR:H48:K-		ND	−
Escherichia hermannii ATCC 33650	NR	Human isolate		−
Escherichia vulneris ATCC 29943		Human wound		−
Shigella boydii ATCC 9207			ND	−
Salmonella enterica serovar Typhimurium NCTC 12416				−
Salmonella enterica serovar Typhi SGSC 3036				−
Salmonella enteritidis SGSC 2476				−
Cronobacter sakazakii CECT 858		Child's throat		−
Cronobacter sakazakii		Milk		−
Klebsiella pneumoniae ATCC 11296				−

^aSGSC, Salmonella Genetic Stock Centre; ATCC, American Type Culture Collection; NCTC, National Collection of Type Cultures; CECT, Spanish Type Culture Collection.

^bE. coli O157 strains that tested negative for the presence of H7 antigen.

^cNT, nontoxigenic E. coli; EPEC, enteropathogenic E. coli (epidemiologically implicated as a pathogen, but the virulence mechanism is not related to the excretion of enterotoxins); ND, not determined; NR, nonrelevant information for the present study.