Fig 4.

Energy requirements in MhpT-mediated 3HPP transport. Assays of 3HPP uptake by IPTG-induced strains W3110ΔmhpT(pVLT31-mhpT) and W3110ΔmhpT(pVLT31) were performed at pH 8.2. ▲, strain W3110ΔmhpT(pVLT31-mhpT) before starvation; ○, starved strain W3110ΔmhpT(pVLT31-mhpT) cells; ■, starved strain W3110ΔmhpT(pVLT31-mhpT) cells reenergized with glucose; ▼, starved strain W3110ΔmhpT(pVLT31-mhpT) cells reenergized with glucose (the addition of CCCP at the 15th second is indicated by an arrow); •, starved CCCP-treated strain W3110ΔmhpT(pVLT31-mhpT) cells reenergized with glucose; □, strain W3110ΔmhpT(pVLT31) before starvation. All points represent the mean values of triplicate trials, with error bars denoting the standard deviation.