Table 2.
GFP variants benchmarked in this study
| GFP | Changes to A. victoria GFP | Properties | Gene codon optimization method | Reference |
|---|---|---|---|---|
| GFPmut1 | F64L, S65T, L195S | 35-fold brighter than wild-type GFP | Original codon adaptation from A. victoria | 18 |
| GFP+ | F64L, S65T, Q80R, F99S, M153T, V163A | 130-fold brighter than wild-type GFP | E. coli | 20 |
| GFP+(htrA) | M1MKHL, F64L, S65T, Q80R, F99S, M153T, V163A | Improved translation efficiency in S. pneumoniae | E. coli | 12 |
| GFP(Sp) | M1MV, S65A, V68L, S72A, A206K | Based on GFPmut2, monomer | S. pneumoniae using OptimumGene | 36 |
| sfGFP(Bs) | S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F, M153T, V163A, I171V, A206V | Superfolder GFP (19) | B. subtilis using dual codon method | This study |
| sfGFP(Sp) | S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F, M153T, V163A, I171V, A206V | Superfolder GFP (19) | S. pneumoniae using OPTIMIZER (55) | This study |
| sfGFP(iGEM) | S2R, S30R, Y39N, F64L, S65T, S72A, F99S, N105T, Y145F, M153T, V163A, I171V, A206V | Superfolder GFP, additional mut3* mutations | E. coli and B. subtilis | 46 |