Table 5.

Mutations in the cardiolipin synthetase gene, cls, identified among daptomycin-nonsusceptible Enterococcus strains

Species and mutation	Predicted Cls domain	Daptomycin MIC (μg/ml)	Reference
Enterococcus faecium
H215R	Proximal to the PLD1 active site (within 10 Å)	48a	164
H270R	Spacer regions between PLD1 and PLD2	32	157
K60T	Linker region	32	160
MPL insertion at 110	Linker region	24a	164
N13I	N-terminal transmembrane helix	32a	164
N13S	N-terminal transmembrane helix	192	157
N13T	N-terminal transmembrane helix	>256	160
R218Q	HKD of PLD1	32a	164
R218Q	HKD of PLD1	48a	164
R218Q	HKD of PLD1	16	162
Enterococcus faecalis
Deletion of K61	Linker region	12	164
R218Q	HKD of PLD1	256	163
N77-Q79 del	Linker region	256	163
R218Q	HKD of PLD1	512	163

^aMIC measured by Etest.