Fig 2.

Charge-engineered lysozyme is nontoxic during repeat dosing. Groups of eight mice received 100 μg of enzyme daily. Experimental groups received a total of one, two, or three doses. Following treatment, four mice from each group were sacrificed for analysis on day 4 and four mice were sacrificed for analysis on day 10. (A) BALF immune cell concentration on day 4 (ANOVA, P = 0.377); (B) BALF immune cell concentration on day 10 (ANOVA, P = 0.192). The means ± SEMs are indicated, and there were no significant differences compared to the results for the PBS control. (C) Liver damage assessed by hematoxylin-eosin staining. Representative images are shown, and no differential pathology was observed between experimental groups and the PBS control. Magnifications, ×100.