Table 2.
Oligonucleotides used in this study
| Name | Sequencea |
|---|---|
| Primer 1 | 5′-GCAGCTCTTGCTCAATCC |
| Primer 2 | 5′-CAAGCGACTTATGGGAGG |
| oppE#1 | 5′-CTGCTGGAGCTCATCGGCGAAGC |
| oppE#2 | 5′-ATGGAGAGCTCGCTGGCGACTCC |
| oppB-up | 5′-GGAGCTCGACAAGGTGGTCGC |
| oppB-down | 5′-GGAGCTCAGTGGCTGGCCGTC |
| oppA-up | 5′-CAAGCTTGGCCAGAACCCCGG |
| oppA-down | 5′-AGGTACCAGTTGGGAATGCTGTAG |
| FabI-2 | 5′-CGCGGGTGGTCACCGCGG |
| OppE-1 | 5′-ATGGCCCCGGCAAGCGCG |
| P244 | 5′-CTGCGGCAACTGGAGGGCAAG |
| P245 | 5′-CCGGGCCCTGAGCTTGTCGTT |
| lacZ-148 | 5′-GGGTAACGCCAGGGTTTTCC |
| Pser-down | 5′-AGTTCGGCCTGGTGGAACAACTCG |
a
Newly introduced SacI (oppE#1 and oppE#2), HindIII (oppA-up), KpnI (oppA-down), and ApaI (P245) restriction sites are underlined.