Table 5.
Characterization of P. aeruginosa transposon and defined opp mutants exhibiting high-level pacidamycin resistance
| Strain | Description | MIC (μg/ml) |
|||
|---|---|---|---|---|---|
| Pacidamycin | Levofloxacin | Carbenicillin | Tetracycline | ||
| PAO1 | Wild type | 16 | 0.25 | 32 | 4 |
| PAM1194 | PAO1 pac::mini-D171b | >512 | 0.25 | 32 | 512a |
| PAM1135 | PAO1 pac-1 | >512 | 0.25 | 32 | ND |
| PAM1316 | PAO1194 oprM::Hgr | >512 | 0.015 | 0.5 | 32 |
| PAM2386 | PAO1 pac::mini-D171b,c | >512 | 1 | 256 | >512a |
| PAO235 | PAO1 fabI::FRT | 32 | 0.25 | ND | ND |
| PAO315 | PAO1 oppE::FRT | >512 | 0.25 | ND | ND |
| PAO1284 | PAO1 ΔoppB::FRT | >512 | 0.25 | ND | ND |
| PAO449 | PAO1 oppA::FRT | 256 | 0.25 | ND | ND |
| PW4183 | PAO1 oppA::ISlacZ/hah | >512 | ND | ND | ND |
| PW4184 | PAO1 oppA::ISlacZ/hah | >512 | ND | ND | ND |
a
The mini-D171 element contains a tetracycline resistance marker.
b
Insertion mapping revealed that in this strain, pac is identical to oppB.
c
The tetracycline resistance marker was transferred from PAM1194 to PAO1 by F116L-mediated transduction.