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Time to heat detection of voriconazole-susceptible (S) (n = 6, circles) and voriconazole-resistant (R) (n = 21, squares) A. fumigatus strains in the presence of serial dilutions of voriconazole from 0 to 8 μg/ml, as measured by isothermal microcalorimetry at 37°C. The numbers indicate the mean detection times in hours. The dashed horizontal line indicates the optimal time (8 h) for distinguishing azole-susceptible and azole-resistant strains in the presence of 0.5 μg/ml of voriconazole.
