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Coomassie blue-stained SDS-PAGE gel showing purified GST-U69. Baculovirus expressing U69 kinase as a GST fusion protein was constructed using the Bac-to-Bac baculovirus expression system from Invitrogen. SF21 insect cells were infected and harvested 65 h postinfection. GST-U69 was purified on a GST column followed by an anion exchange column. Lane 1, molecular weight markers (SeeBluePlus2; Invitrogen); lane 2, purified GST-U69.
