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Replication kinetics in CBMCs of WT and mutant viruses. Viruses were harvested from transfection of HEK293T cells and were used to infect CBMCs through normalization of the p24 antigen level of the inoculum. Virus production was monitored at the indicated time points by measuring p24 levels in cell-free supernatants by ELISA. Each experiment was performed in triplicate. The results are representative of those from two independent experiments.
