Fig 2.

The nongrowing ΔmvaS mutant is tolerant toward multiple bactericidal antibiotics. The CFU/ml of wt HG001, the mevalonate-depleted ΔmvaS mutant, and the complemented mutant after 6 h of incubation with various bactericidal antibiotics is shown: gentamicin (A), rifampin (B), ciprofloxacin (C), and daptomycin (D). The dotted lines indicate the starting CFU/ml (≈10⁷) before antibiotics were added. The resuscitation experiment where the ΔmvaS mutant was cultivated with 960 μM mevalonate is shown in panel A with gentamicin. White bar, without antibiotics; light-gray bar, addition of 1× MIC antibiotics; dark-gray bar, addition of 10× MIC antibiotics. Error bars represent the standard deviations from three independent experiments.