Fig 2.

ERG11 expression. (A) ERG11/ACT1 mRNA ratios did not correlate with log₂ fluconazole MICs in 25 Pacific Northwest C. gattii, 34 non-Pacific Northwest C. gattii, and 20 C. neoformans clinical isolates. (B) The ERG11/ACT1 mRNA ratios were higher when 4 strains with the highest and the 4 strains of Pacific Northwest C. gattii, non-Pacific Northwest C. gattii, or C. neoformans with the lowest fluconazole MICs were incubated in the presence of 8 μg/ml fluconazole for 2 h (gray bars) than in cells incubated in the absence of fluconazole (white bars), but there were no significant differences in ERG11/ACT1 mRNA ratios between the 3 groups or between the 4 strains with the high and low fluconazole MICs within each group. Shown are geometric means ± standard deviations from 3 experiments.