Table 1.
Analysis and gene | Orientation | Primer sequence (5′–3′) | UPL probea | Reference or source |
---|---|---|---|---|
RT-PCR analysis | ||||
rpoB | Forward | CGTGTATCTGCGCTTGG | 131 | This study |
Reverse | CGTACTTCGAAGCCTGCAC | |||
gyrB | Forward | TGGTGGAACGTGGTCATATTT | 76 | This study |
Reverse | TGCTCTTGCTTACCCTTTTTG | |||
adeB | Forward | CGAGTGGCACAACTAGCATC | 61 | This study |
Reverse | CCTTGTCTTGGCTGCACTCT | |||
adeJ | Forward | CCTATTGCACAATATCCAACGA | 119 | This study |
Reverse | AGGATAAGTCGCAGCAATCG | |||
adeG | Forward | GTCCTGAAATGGTCGTTCGT | 43 | This study |
Reverse | AGCTTCTGCTTGGCTAGATGA | |||
craA | Forward | TTCATTGCTTGCGCCTTT | 125 | This study |
Reverse | CCAGTGCCATGAAACATAATCA | |||
abeM | Forward | AGGGACGTATTATGGCGAAA | 165 | This study |
Reverse | CTGCTGTGCTTAGACCAATTTTT | |||
amvA | Forward | GCAGAGAAATTTTGCACTTGG | 10 | This study |
Reverse | CGACTAATGGACCAAAAGCTG | |||
ompA | Forward | GGTATTCAGATAATTTTTCAGCAACTT | 129 | This study |
Reverse | AACAAATCAAACATCAAAGACCAA | |||
ompW | Forward | GCCTTATTTGCTCTGCCAAC | 60 | This study |
Reverse | CGTTTGAAACCATCACCATCT | |||
dcap-like | Forward | TGATCGACTTCTCGACAAACA | 77 | This study |
Reverse | GTGTAGTTGGGCCTAGTTTGTAGTT | |||
oprC | Forward | ACTCGATACAAAGCGGTGGA | 9 | This study |
Reverse | TTTAATACGTGAACCAAACATACCTC | |||
oprB | Forward | GCCCCACACTTCTTGAACAG | 67 | This study |
Reverse | ATGGGCAATCGCTTTCTG | |||
omp25 | Forward | CGAACGTGAAATCGACAACA | 128 | This study |
Reverse | CGTAACCTTTAACACCTAGAGCAAG | |||
omp33-omp36 | Forward | CAAGTGTTGCTAACCAATTCGCT | FAM-CCAAACTGCTGCTATCCAAAACGACCAA-BBQ | This study |
Reverse | GTTTTCTTGACCGAATGCACC | |||
carO | Forward | TGTTCATGACAGCTATGCATTCGATA | FAM-CGCTCGTGCTGAAGTAGGTACTACAGGTT-BBQ | This study |
Reverse | CCCAATGCTAAACCTACATATGGGT | |||
Sequencing analysis | ||||
adeR | Forward | ACTACGATATTGGCGACATT | ||
Reverse | GCGTCAGATTAAGCAAGATT | 13–15 | ||
adeS | Forward | TTGGTTAGCCACTGTTATCT | ||
Reverse | AGTGGACGTTAGGTCAAGTT | 13–15 | ||
adeN | Forward | GCTGTTAGGTTGGGGTCGTA | ||
Reverse | CGTGACCAAAAGTACGAATCA | 18 |
FAM, 6-carboxyfluorescein; BBQ, BlackBerry Quencher.