Table 4.

MICs and RE of efflux pumps^c

Straina	MICd (mg/liter)										REb
	TIG	GEN	AMK	DOX	MIN	TET	NET	RIF	TOB	DOR	adeB	adeJ	adeG	craA	abeM	amvA
421	1	>64	64	<0.5	<0.5	4	64	2	64	1	NA	1.44	1.23	1.20	0.04	0.01
422	1	16	16	<0.5	<0.5	8	8	2	8	<0.5	NA	1.37	2.11	0.82	0.05	0.02
423	1	16	16	<0.5	<0.5	8	8	2	16	1	NA	1.21	1.16	0.63	0.07	0.02
424	1	16	64	1	1	4	8	16	32	<0.5	NA	1.41	0.89	0.68	0.04	0.02
426	1	64	32	1	1	8	64	16	32	<0.5	NA	1.69	1.00	0.45	0.04	0.02
427	2	16	16	8	<0.5	>64	8	4	8	1	NA	1.46	0.83	0.42	0.04	0.02
428	2	16	16	4	<0.5	>64	4	2	8	1	NA	1.64	0.89	0.49	0.05	0.02
429	2	64	4	4	<0.5	>64	8	4	8	1	NA	1.75	0.91	0.48	0.04	0.01
430	2	32	16	8	<0.5	>64	16	4	16	1	NA	1.22	0.76	0.48	0.04	0.02
431	2	64	4	8	1	>64	32	32	32	1	NA	1.28	1.00	0.44	0.04	0.02
432	2	16	16	4	<0.5	>64	4	4	16	2	NA	1.61	0.90	0.53	0.04	0.02
433	2	16	16	8	<0.5	>64	8	4	16	2	NA	1.51	0.89	0.54	0.04	0.02
434	2	16	16	4	<0.5	>64	8	4	8	2	NA	1.82	1.09	0.50	0.04	0.02
435	2	32	32	4	<0.5	>64	8	2	32	1	NA	1.27	0.90	0.34	0.05	0.01
436	2	16	16	8	1	>64	16	4	8	1	NA	1.32	0.79	0.43	0.04	0.01
437	2	16	16	4	1	>64	4	4	16	<0.5	NA	0.72	1.18	0.78	0.03	0.03
438	2	32	16	8	1	>64	8	4	16	<0.5	NA	1.25	1.51	0.60	0.03	0.02
443	2	16	16	4	<0.5	>64	8	4	8	1	NA	0.91	0.80	0.52	0.04	0.01

^aStrains are ranked according to the MIC of tigecycline.

^bPrimers for adeA and adeC genes were also used. NA, not applicable.

^cThe reference strain used was A. baumannii ATCC 17978. RNA (50 μg/ml) was from strains of the PFGE-HUI-1 clone (susceptible to carbapenems).

^dTIG, tigecycline; GEN, gentamicin; AMK, amikacin; DOX, doxycycline; MIN, minocycline; TET, tetracycline; NET, netilmicin; RIF, rifampin; TOB, tobramycin; DOR, doripenem.