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H2O2 activation of Yap1-EGFP in axenic culture. An E. festucae wild-type strain (PN2874) expressing S. cerevisiae Yap1-EGFP was examined by confocal laser scanning and DIC microscopy in the absence of oxidative stress (PD) or 30 min after treatment with 16 mM H2O2 or 4 mM diamide. EGFP images were generated by maximum-intensity projection of confocal z stacks taken at 1-μm intervals from the top to the bottom of the section. Bars = 20 μm.
