Table 1.
Summary of fermentation results for the C. beijerinckii knockout mutants compared to the wild-type straina
| Characteristic | Value in: |
|||||
|---|---|---|---|---|---|---|
| P2 medium |
MP2 medium |
|||||
| WT | pta | buk | WT | pta | buk | |
| Growth rate (/h) | 0.13 | 0.11 | 0.12 | 0.11 | 0.06 | 0.07 |
| Butanol (g/liter) | 9.9 | 9.1 | 12.7 | 10.6 | 12.4 | 12.2 |
| Butanol yield (g/g of glucose) | 0.25 | 0.28 | 0.24 | 0.22 | 0.23 | 0.23 |
| Acetone (g/liter) | 4.4 | 3.4 | 5.6 | 4.2 | 5.3 | 4.9 |
| Ethanol (g/liter) | 0.2 | 0.1 | 0.3 | 0.4 | 0.4 | 0.4 |
| Total ABE (g/liter)b | 14.5 | 12.6 | 18.6 | 15.2 | 18.1 | 17.5 |
| ABE yield (g/g of glucose) | 0.37 | 0.38 | 0.35 | 0.32 | 0.34 | 0.33 |
| ABE productivity (g/liter/h) | 0.18 | 0.20 | 0.42 | 0.19 | 0.25 | 0.24 |
| Peak acetic acid (g/liter)c | 1.64 | 1.39 | 1.81 | 1.01 | 0.51 | 1.00 |
| Peak butyric acid (g/liter) | 1.00 | 1.53 | 0.97 | 0.76 | 0.88 | 0.80 |
| Peak lactic acid (g/liter) | 0.08 | 0.13 | 0.02 | 0.18 | 0.60 | 0.17 |
| Carbon recovery (%) | 87.9 | 91.6 | 87.1 | 79.5 | 86.1 | 85.6 |
a
WT, wild type; pta, pta knockout mutant [C. beijerinckii pta::int(17)]; buk, buk knockout mutant [C. beijerinckii buk::int(532)].
b
The reported titers were the maximum values after the solvent production reached plateau.
c
There was approximately 1.3 g/liter of acetic acid preadded in the P2 medium.