Table 2.
Enzyme activities of C. beijerinckii knockout mutants and the wild-type strain
| Straina | Sp act (U/mg of protein)b |
|||
|---|---|---|---|---|
| Pta | Ack | Ptb | Buk | |
| WT | 24.4 ± 3.9 | 0.15 ± 0.02 | 12.6 ± 1.6 | 0.12 ± 0.01 |
| pta | 5.8 ± 0.6 | 0.07 ± 0.01 | 15.8 ± 2.5 | 0.13 ± 0.02 |
| buk | 21.2 ± 0.3 | 0.10 ± 0.01 | 11.2 ± 1.6 | 0.04 ± 0.01 |
a
WT, wild type; pta, pta knockout mutant [C. beijerinckii pta::int(17)]; buk, buk knockout mutant [C. beijerinckii buk::int(532)].
b
Pta, phosphotransacetylase; Ack, acetate kinase; Ptb, phosphate butyryltransferase (phosphotransbutyrylase); Buk, butyrate kinase.