Table 3.
Calculated substrate uptake and product secretion rates in different strains based on the experimental MP2 fermentation results
| Straina | Calculated flux rate,b mmol/g (dry wt)/h |
|||||
|---|---|---|---|---|---|---|
| Glucose | Acetate | Butyrate | Acetone | Butanol | Ethanol | |
| WT | −10.1 | 2.68 | 2.49 | 3.23 | 5.87 | 0.30 |
| pta | −7.16 | 0.90 | 1.38 | 2.32 | 4.22 | 0.25 |
| buk | −6.71 | 1.76 | 1.40 | 2.37 | 4.11 | 0.21 |
a
WT, wild type; pta: pta knockout mutant [C. beijerinckii pta::int(17)]; buk, buk knockout mutant [C. beijerinckii buk::int(532)].
b
Experimentally observed flux rates for growth, glucose, acetate, butyrate, acetone, butanol, and ethanol (a negative rate depicts an uptake rate). Rates were calculated only for fermentation on MP2 medium, as this medium lacks acetate and therefore gives a clear picture of the acidogenesis phase.