Table 1.
Target genes and primer sequences for the PCR detection of EPEC gene markers
| Target gene or plasmid and primer | Sequence (5′→3′) | Amplicon size (bp) | Reference |
|---|---|---|---|
| Genes | |||
| stx1 | |||
| Stx1F | ATAAATCGCCATTCGTTGACTAC | 180 | 24 |
| Stx1R | AGAACGCCCACTGAGATCATC | ||
| stx2 | |||
| Stx2F | GGCACTGTCTGAAACTGCTCC | 255 | |
| Stx2R | TCGCCAGTTATCTGACATTCTG | ||
| eae | |||
| SK1 | CCCGAATTCGGCACAAGCATAAGC | 881 | 25 |
| SK2 | CCCGGATCCGTCTCGCCAGTATTCG | ||
| bfpA | |||
| EP1 | ATTGGTGCTTGCGCTTGCTGC | 326 | 27 |
| EP2 | GCCGCTTTATCCAACCTGGTA | ||
| Plasmid | |||
| pEAF | |||
| EAF1 | CAGGGTAAAAGAAAGATGATAA | 397 | 26 |
| EAF25 | TATGGGGACCATGTATTATCA |