Fig 1.

Leucine and phenylalanine release by isolated Buchnera preparations. (A) Metabolic map of the terminal reactions for leucine and phenylalanine biosynthesis (shaded box represents Buchnera). (B) Western blot of 5 μg of protein from whole aphid (WB) and bacteriocytes (BC) using polyclonal antibody against BCAT. (C) Western blot of 5 μg of protein from WB and BC using polyclonal antibodies against GOT2. (For panels B and C, molecular masses are shown to the right [34 to 72 kDa].) (D) Leucine release from Buchnera with and without HF and release from Buchnera with and without recombinant IlvE. (E) Phenylalanine release from Buchnera with and without HF and release from Buchnera with and without recombinanat TyrB. ht, heat treated. Values are means ± SEs. P < 0.01 by LSD post hoc test (different letters refer to significantly different mean values).