Fig 2.

(A) Native PAGE analysis of recombinant rPinA614 harvested at 4, 5, 6, 7, 8, 9, and 24 h. The metastable (rPinA614) and latent (rPinA614-L) forms are indicated. The identity of rPinA614 and rPinA614-L from 5 to 24 h was confirmed by LC-EIS MS/MS. (B) SDS-PAGE analysis of recombinant rPinA614 harvested at 5, 6, 7, 8, 9, and 24 h. (C) Native PAGE analysis of the thermostability profile of rPinA614 heated at 70°C and harvested as indicated. The identity of rPinA614 and rPinA614-L at all time points was confirmed by LC-EIS MS/MS with the exception of rPinA614-L at 60 min. (D) Thermostability analysis of rPinA75 and rPinA614. The thermal stability of rPinA was determined essentially as described by Kang et al. (2) except that the reactions were performed at 60°C. The thermostability analyses were independently performed at least twice per sample.