Fig 3.

Penicillin titers and mRNA steady-state levels of penicillin biosynthesis genes of histone H3 mutant strains. Wild-type and histone mutant strains were grown in fermentation media and harvested. (A) Penicillin titers measured after 36 h are given as relative values, with the amount measured for the wild type set at 100%. Statistical significance of data is given by the P value (**, P < 0.01; ***, P < 0.001). (B) Fold difference of the mRNA steady-state level of penicillin biosynthesis genes ipnA and aatA and the nonrelated gene ANIA_2647 measured by qRT-PCR after 12 h of incubation of strains. The β-actin gene of A. nidulans was used as an internal standard. Statistical significance of data is given by the P value (*, P < 0.05; **, P < 0.01).