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SDS-PAGE analysis of expression of mimA and mimC in E. coli. Samples prepared from E. coli cells carrying pRSFDuet-1 and pCDFDuet-1 (empty vector), pRSFDmimAC and pCDFDuet-1 (mimAC), pRSFDmimAC and pCDFDmimG (mimAC + mimG), or pRSFDmimAC and pCDFDmimG-groES (mimAC + mimG + groES) were loaded onto a polyacrylamide gel (7.5%). S, soluble-fraction sample; W, whole-cell sample. The molecular masses corresponding to MimA, MimC, and MimG are indicated by arrows.
