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SDS-PAGE analysis of expression of mimB and mimD in E. coli. Samples prepared from E. coli cells carrying pETDuet-1 (empty vector), pETDmimBD (mimBD), pETDmimBDop (mimBDop), or pETDmimB5′opDop (mimB5′opDop) were loaded onto a polyacrylamide gel (15%). S, soluble-fraction sample; W, whole-cell sample. The molecular masses corresponding to MimB and MimD are indicated by arrows.
