Table 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Characteristicsa | Reference(s) or source |
|---|---|---|
| Strains | ||
| E. coli JM109 | Host used for cloning | TaKaRa Bio |
| E. coli Rosetta 2(DE3)pLysS | Host used for expression | Novagen |
| Plasmids | ||
| pETmimABCDgo | pET21a containing mimABCDgo | 20, 21 |
| pTipmimGsm | pTip-RT2 containing mimGsm | 20 |
| pRSFDuet-1 | Vector used for cloning, RSF origin, Knr | Novagen |
| pCDFDuet-1 | Vector used for cloning, CDF origin, Smr | Novagen |
| pETDuet-1 | Vector used for cloning, pBR322 origin, Apr | Novagen |
| pRSFDmimA | pRSFDuet-1 containing mimA in MCS-1 under the control of the T7 promoter | This study |
| pRSFDmimAC | pRSFDuet-1 containing mimA in MCS-1 and mimC in MCS-2 under the control of the T7 promoters | This study |
| pCDFDmimG | pCDFDuet-1 containing mimG in MCS-1 under the control of the T7 promoter | This study |
| pCDFDmimG-groES | pCDFDuet-1 containing mimG in MCS-1 and groES in MCS-2 under the control of the T7 promoters | This study |
| pETDmimB | pETDuet-1 containing mimB in MCS-1 under the control of the T7 promoter | This study |
| pETDmimBD | pETDuet-1 containing mimB in MCS-1 and mimD in MCS-2 under the control of the T7 promoters | This study |
| pETDmimDop | pETDuet-1 containing mimDop in MCS-2 under the control of the T7 promoter | This study |
| pETDmimBDop | pETDuet-1 containing mimB in MCS-1 and mimDop in MCS-2 under the control of the T7 promoters | This study |
| pETDmimB5′opDop | pETDuet-1 containing mimB5′op in MCS-1 and mimDop in MCS-2 under the control of the T7 promoters | This study |
a
Knr, kanamycin resistance; Smr, streptomycin resistance; Apr, ampicillin resistance.