Table 1.

Primers and probes used in this study

No.	Primer	Sense or probe	5′–3′ sequencea	Function
1	ro04074	Forward	TGG TCA GGA TGC AGG GAG TT	Primers and probes for quantification of porin genes by RT-QPCR
2	ro04074	Probe rpJA	TGG CTC CAT CTC C
3	ro04074	Reverse	GTG ACC GGC CCG ATA GC
4	ro03127	Forward	GAG GCA ACC GGG TCG AA
5	ro03127	Probe rpJB	TCC TGC AAG GTG ATA CC
6	ro03127	Reverse	CGG CGG GAC CAC TTG ATA C
7	ro03156	Forward	CAG TGG CTC AAC GAC GTG AT
8	ro03156	Probe rpJC	CTC AAC GGC ACC CC
9	ro03156	Reverse	TGG ACT TGA TCG CGG TGT T
10	ro08561	Forward	CCG CCG CTG GAT GGT
11	ro08561	Probe rpJD	TTC CCA CCA GTG TCG AG
12	ro08561	Reverse	GGC GTA ACC GTT GTG GAA GA
13	ro04074_P1	Forward	CCCAAGCTTAGGCGATCAGCAAGCCGAGGACG	Upstream flanking region amplification primers
14	ro04074_P2	Reverse	TGCTCTAGACTGCGACTGCCACCGAGCGTG
15	ro04074_P3	Forward	TGCTCTAGAGGCCGTGACGGAGAACGGTG	Downstream flanking region amplification primers
16	ro04074_P4	Reverse	CGCGGATCCCGGTGTGCTGGGCGCG
17	ro04074_P7	Forward	CGGATTCGAGGAGCGGCAGGTG	rjpA deletion confirmation primers
18	ro04074_P8	Reverse	GGCGGTGAGGTGGTG GTGCATG
19	ro04074_P9	Forward	GGAATTCCATATGGGTCGGCATGACGGACATCAGC	Primers to amplify rjpA for complementation
20	ro04074_P10	Reverse	CCCAAGCTTCTAGTTGAGCTTCTGCGGCACACCG

^aRestriction sites are underlined.