Table 2.

Reaction efficiencies, average quantification cycle (${\mathrm{C}}_{\mathrm{q}}$) values, average expression ratios calibrated to 28°C, and standard deviations of average expression ratios of rpoE under different stress conditions at the early and late logarithmic growth phases^ac

Condition	E	Early logarithmic growth phase				Late logarithmic growth phase
		$\overline{\mathit{X}}\left({\mathrm{C}}_{\mathrm{q}}\right)$b	$\overline{\mathit{X}}\left(\mathrm{R}\right)$c	SD (R)	P value	$\overline{\mathit{X}}\left({\mathrm{C}}_{\mathrm{q}}\right)$b	$\overline{\mathit{X}}\left(\mathrm{R}\right)$c	SD (R)	P value
3°C	0.86	14.17	3.2	0.6	0.007	15.02	2.6	1.7	0.184
37°C	0.95	15.28	2.2	0.3	0.011	16.21	2.2	0.4	0.008
42°C	0.99	14.27	3.9	0.4	0.002	16.24	1.8	0.3	0.035
pH 5.0	1.01	18.43	13.0	4.3	0.007	20.74	3.5	3.2	0.235
pH 9.0	0.90	19.17	3.9	0.6	0.005	21.22	0.9	0.5	0.599
3% NaCl	0.97	20.01	7.2	1.6	0.004	22.22	1.1	0.8	0.737
3% ethanol	0.94	16.85	8.5	3.0	0.010	16.65	13.1	13.4	0.102

^aE, reaction efficiencies; R, average expression ratios.

^bAverage of the reverse transcription replicates, PCR replicates, and biological replicates.

^c$\mathrm{R}=\frac{{(1+{\mathrm{E}}_{rpoE})}^{{\mathrm{\Delta }\mathrm{C}}_{\mathrm{q},rpoE}}}{{(1+{\mathrm{E}}_{16\mathrm{S}rrn})}^{{\mathrm{\Delta }\mathrm{C}}_{\mathrm{q},16\mathrm{S}rrn}}}$, where ${\mathrm{\Delta }\mathrm{C}}_{\mathrm{q}}=\overline{\mathit{X}}\left({\mathrm{C}}_{\mathrm{q},28°\mathrm{C}}\right)-\overline{\mathit{X}}\left({\mathrm{C}}_{\mathrm{q},\mathrm{s}\mathrm{t}\mathrm{r}\mathrm{e}\mathrm{s}\mathrm{s}}\right)$.