Table 3.

Stability of the reference gene 16S rrn at different stress conditions at early and late logarithmic growth phases in Yersinia pseudotuberculosis IP32953

Parameters	Value at:			Ea
	Early logarithmic growth phase	Late logarithmic growth phase	Both growth phases
3°C and 28°C				1.05
Arithmetic mean (Cq)	16.91	17.00	16.96
Geometric mean (Cq)	16.90	17.00	16.95
Avg deviation	0.63	0.30	0.46
CV (% Cq)b	3.89	2.00	3.10
37°C and 28°C				0.94
Arithmetic mean (Cq)	18.40	18.27	18.34
Geometric mean (Cq)	18.40	18.26	18.33
Avg deviation	0.25	0.20	0.24
CV (% Cq)b	1.60	1.23	1.48
42°C and 28°C				0.95
Arithmetic mean (Cq)	17.83	17.64	17.74
Geometric mean (Cq)	17.83	17.63	17.73
Avg deviation	0.23	0.31	0.27
CV (% Cq)b	1.52	2.08	1.90
pH 5.0 and 28°C				1.04
Arithmetic mean (Cq)	13.97	13.84	13.90
Geometric mean (Cq)	13.96	13.82	13.89
Avg deviation	0.35	0.53	0.44
CV (% Cq)b	2.84	4.28	3.65
pH 9.0 and 28°C				0.93
Arithmetic mean (Cq)	18.38	18.13	18.25
Geometric mean (Cq)	18.36	18.11	18.24
Avg deviation	0.71	0.72	0.73
CV (% Cq)b	4.07	4.35	4.26
3% NaCl and 28°C				0.94
Arithmetic mean (Cq)	18.98	18.48	18.73
Geometric mean (Cq)	18.94	18.45	18.69
Avg deviation	1.29	1.04	1.16
CV (% Cq)b	6.85	5.78	6.49
3% ethanol and 28°C				0.95
Arithmetic mean (Cq)	17.91	17.78	17.85
Geometric mean (Cq)	17.90	17.77	17.84
Avg deviation	0.46	0.56	0.51
CV (% Cq)b	2.88	3.35	3.14

^aE, reaction efficiency.

^bCV, coefficient of variance as a percentage of the C_q value.