Table 4.
Genes significantly differentially expressed in Lb. delbrueckii subsp. bulgaricus in response to cocultivation with S. cerevisiae for the enriched functional categories amino acid transport and metabolism and lipid metabolisma
| Functional category and locus tag | Description | Fold change in expression for cocultures vs monocultures |
|---|---|---|
| Amino acid transport and metabolism (14 genes)b | ||
| LBUL_0215 | ABC-type amino acid transport system permease component | −2.7 |
| LBUL_0216 | ABC-type polar amino acid transport system ATPase component | −3.6 |
| LBUL_0737 | Transcriptional regulators containing a DNA-binding helix-turn-helix domain and an aminotransferase domain (MocR family) and their eukaryotic orthologs | −15.3 |
| LBUL_0915 | Dipeptidyl aminopeptidases/acylaminoacyl peptidases | 12.1 |
| LBUL_1181 | Diaminopimelate decarboxylase | 2.2 |
| LBUL_1235 | Cysteine synthase | −2.3 |
| LBUL_1236 | Cystathionine beta-lyases/cystathionine gamma-synthases | −2.4 |
| LBUL_1257 | Aspartate semialdehyde dehydrogenase | −4.0 |
| LBUL_1353 | Homoserine trans-succinylase | −2.3 |
| LBUL_1619 | Acetylornithine deacetylase/succinyl-diaminopimelate desuccinylase and related deacylases/dipeptidase PepV | −2.5 |
| LBUL_1630 | Homoserine kinase | −2.3 |
| LBUL_1631 | Threonine synthase | −2.5 |
| LBUL_1646 | Amino acid transporters | −4.0 |
| LBUL_1668 | Gamma-aminobutyrate permease and related permeases | −9.0 |
| Lipid metabolism (10 genes)c | ||
| LBUL_0784 | Predicted acyltransferases | 5.1 |
| LBUL_0806 | 3-Hydroxy-3-methylglutaryl coenzyme A synthase | −3.0 |
| LBUL_0818d | 3-Oxoacyl-(acyl carrier protein) | 3.0 |
| LBUL_0819d | Acyl carrier protein | 3.8 |
| LBUL_0821d | 3-Oxoacyl-(acyl carrier protein) reductase/dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) | 2.2 |
| LBUL_0822d | 3-Oxoacyl-(acyl carrier protein) synthase | 2.8 |
| LBUL_0823d | Biotin carboxyl carrier protein | 3.3 |
| LBUL_0824d | 3-Hydroxymyristoyl/3-hydroxydecanoyl-(acyl carrier protein) dehydratases | 3.3 |
| LBUL_0825d | Acetyl/propionyl coenzyme A carboxylase alpha subunit | 2.6 |
| LBUL_1607d | 3-Hydroxymyristoyl/3-hydroxydecanoyl-(acyl carrier protein) dehydratases | −2.5 |
a
Differential expression was identified by use of a false discovery rate of <5% (see Materials and Methods). The complete list of enriched categories is available in Table S2 in the supplemental material.
b
The genome contained 109 genes in this category.
c
The genome contained 39 genes in this category.
d
Genes in the long-chain fatty acid production pathway.