Table 2.

Effects of metal ions and chemical agents on the activity of purified recombinant BglQM

Metal ion and reagent	Relative activity ± SD (%) at:
	1 mM	10 mM
NaCl	91.1 ± 1.27	93.6 ± 3.14
KCl	92.0 ± 5.78	90.9 ± 1.61
MgCl2	93.8 ± 3.18	85.0 ± 2.52
CaCl2	99.6 ± 2.55	102.5 ± 5.21
CoCl2	92.9 ± 1.68	76.1 ± 1.35
CuCl2	46.4 ± 0.95	NDa
SDS	84.9 ± 2.26	16.5 ± 0.62
EDTA	64.3 ± 1.36	59.5 ± 0.87
Beta-mercaptoethanol	94.6 ± 2.22	95.4 ± 3.58
Control	100.0 ± 2.36	100.0 ± 2.36

^aND, not determined.