Fig 3.

Hydrolysis of N7-Pan is inhibited RR6. (A) Mass spectrometric analysis of a sample containing 1% serum and N7-Pan after 24 h incubation showed a heptylamine peak but no N7-Pan-peak. Note that the produced pantothenic acid is only partially visualized with the settings used. (B) Pantetheinase inhibitor RR6 is able to protect N7-Pan against hydrolysis, as clear peaks corresponding to the N7-Pan adducts are shown on the mass spectrum. Also, the RR6 adducts and a very small peak corresponding to heptylamine were detected. The reaction was performed as described for panel A.