Table 1.
Resistance and cross-resistance characterization of the R0.8 bloodstream form cell line, compared to the parental wild-type T. brucei brucei strain Lister 427
| Compound | Average EC50 (μM) |
Resistance factor | P valuea | |
|---|---|---|---|---|
| Lister 427 | R0.8 | |||
| PDE inhibitors | ||||
| CpdA | 0.08 ± 0.01 | 1.4 ± 0.2 | 17.2 | 0.004 |
| CpdB | 0.13 ± 0.03 | 1.28 ± 0.25 | 9.7 | 0.016 |
| Dipyridamole | 17.9 ± 2.7 | 9.2 ± 0.8 | 0.5 | 0.059 |
| cAMP analogues | ||||
| Dibutyryl cAMP | 263 ± 13 | 1890 ± 314 | 7.2 | 0.011 |
| 8-Bromo-cAMP | 271 ± 8 | 1133 ± 185 | 4.2 | 0.014 |
| 8-(4-chlorophenylthio)-cAMP | 1.24 ± 0.4 | 0.25 ± 0.05 | 0.2 | 0.201 |
| Known trypanocides | ||||
| Suramin | 0.0212 ± 0.0008 | 0.0156 ± 0.0005 | 0.7 | 0.001 |
| Diminazene | 0.022 ± 0.007 | 0.011 ± 0.001 | 0.5 | 0.133 |
| Pentamidine | 0.0016 ± 0.0004 | 0.0014 ± 0.0002 | 0.9 | 0.683 |
| Cymelarsen | 0.0038 ± 0.0004 | 0.0038 ± 0.0003 | 1.0 | 1.000 |
| Phenylarsine oxide | 0.00083 ± 0.00006 | 0.00088 ± 0.00011 | 1.1 | 0.783 |
| Nifurtimox | 2.01 ± 0.24 | 1.61 ± 0.08 | 0.8 | 0.246 |
a
For comparison of R0.8 and Lister 427.