Table 3.
Alterations of transcription for ΔIP-H5 versus N315ΔIP and N315 rpoB(N967I) versus N315
| Function and ORFa | Gene | Productb | Ratio of signal intensity (mutant to parent strain) |
|
|---|---|---|---|---|
| ΔIP-H5/N315ΔIP | N315rpoB(N967I)/N315 | |||
| Environmental information processing | ||||
| Autolysin-associated genes and regulators | ||||
| SA0252 | lrgA | Holin-like protein; murein hydrolase regulator LrgA | 2.53 | 6.57 |
| SA0253 | lrgB | Antiholin-like protein LrgB | 2.08 | 6.60 |
| SA2327 | cidC | Regulatory protein for murein hydrolase activity | 0.44 | 0.26 |
| SA2328 | cidB | Regulatory protein for murein hydrolase activity | 0.48 | 0.27 |
| SA2329 | cidA | Regulatory protein for murein hydrolase activity | 0.42 | 0.25 |
| SA1898 | sceD-like | Similar to SceD protein, predicted to be a lytic transglycosylase | 2.21 | 3.26 |
| Other signal transduction and regulators | ||||
| SA2090 | rsr | Repressor of sarR and agr genes | 0.30 | 0.38 |
| SA2091 | sarY | Staphylococcal accessory regulator Y | 0.22 | 0.31 |
| SA2092 | Similar to transcription regulator AraC type regulator | 0.20 | 0.35 | |
| Metabolism | ||||
| Cell-wall biosynthesis and metabolism | ||||
| SA0124 | Similar to glycosyltransferase TuaA | 0.39 | 0.43 | |
| SA0125 | Similar to exopolysaccharide G (EpsG) | 0.35 | 0.40 | |
| SA0523 | Similar to poly(glycerol-phosphate) alpha-glucosyltransferase | 2.03 | 2.33 | |
| SA1964 | fmtB (mrp) | Cell surface protein; inactivation reduces MET resistance | 0.24 | 0.29 |
| Cell envelope biogenesis | ||||
| SA0126 | Similar to capsular polysaccharide synthesis protein 14H | 0.39 | 0.43 | |
| SA0127 | Similar to capsular polysaccharide synthesis protein 14L | 0.47 | 0.50 | |
| Carbon hydrate metabolism | ||||
| Nitrogen metabolism | ||||
| Energy metabolism | ||||
| Nucleotide transport and metabolism | ||||
| SA1047 | pyrF | Orotidine-5-phosphate decarboxylase | 0.34 | 0.48 |
| SA1172 | Similar to GMP reductase | 0.32 | 0.47 | |
| Sugar transport and metabolism | ||||
| SA0837 | Similar to 2-isopropylmalate synthase | 2.95 | 2.86 | |
| SA1991 | lacG | 6-Phospho-beta-galactosidase | 0.29 | 0.23 |
| SA1992 | lacE | PTS system, lactose-specific IIBC component | 0.29 | 0.25 |
| SA1993 | lacF | PTS system, lactose-specific IIA component | 0.34 | 0.19 |
| SA1994 | lacD | Tagatose-1,6-diphosphate aldolase | 0.36 | 0.21 |
| SA1995 | lacC | Tagatose-6-phosphate kinase | 0.42 | 0.21 |
| SA1996 | lacB | Galactose-6-phosphate isomerase LacB subunit | 0.49 | 0.25 |
| SA2486 | 2-Oxoglutarate/malate translocator homolog | 2.13 | 2.03 | |
| Amino acid transport and metabolism | ||||
| SA0822 | argG | Argininosuccinate synthase | 0.40 | 0.48 |
| SA0850 | Similar to oligopeptide ABC transporter oligopeptide-binding protein | 0.27 | 0.44 | |
| Terpenoid metabolism | ||||
| SA1304 | Similar to component A of hexaprenyl diphosphate synthase | 2.24 | 2.19 | |
| Secretion and other transport system | ||||
| SA0956 | Similar to Mn2+ transport protein | 0.20 | 0.32 | |
| SA2203 | mdeA | Similar to multidrug resistance protein | 2.36 | 2.14 |
| SA2442 | secA2 | Preprotein translocase secA homolog | 2.31 | 2.18 |
| SA2443 | asp3 | Accessory secretory protein Asp3 | 3.10 | 3.20 |
| SA2444 | asp2 | Accessory secretory protein Asp2 | 3.89 | 3.36 |
| SA2445 | asp1 | Accessory secretory protein Asp1 | 5.93 | 4.04 |
| SA2446 | secY2 | Similar to preprotein translocase secY | 5.51 | 4.01 |
| Genetic information processing | ||||
| SA0706 | Similar to comF operon protein 3 | 2.34 | 3.16 | |
| SA1899 | Similar to single-strand DNA binding protein | 2.41 | 2.56 | |
| Virulence factor | ||||
| SA0393 | ssl11 | Exotoxin 15 | 0.31 | 0.23 |
| SA0519 | sdrC | Ser-Asp-rich fibrinogen-binding, bone sialoprotein-binding protein | 2.59 | 3.48 |
| SA0520 | sdrD | Ser-Asp-rich fibrinogen-binding, bone sialoprotein-binding protein | 2.26 | 3.45 |
| SA0521 | sdrE | Ser-Asp-rich fibrinogen-binding, bone sialoprotein-binding protein | 4.45 | 4.60 |
| SA1267 | ebhA | Extracellular matrix-binding protein EbhA | 0.22 | 0.11 |
| SA1577 | sasC | S. aureus surface protein C | 0.36 | 0.40 |
| SA2423 | clfB | Clumping factor B | 4.58 | 2.49 |
| SA2430 | aur | Zinc metalloproteinase aureolysin | 0.40 | 3.50 |
| Hypothetical protein | ||||
| SA0262 | Hypothetical protein | 2.18 | 2.35 | |
| SA0266 | Conserved hypothetical protein | 2.17 | 2.16 | |
| SA0267 | Hypothetical protein | 2.10 | 2.21 | |
| SA0268 | Hypothetical protein | 2.07 | 2.74 | |
| SA0269 | Hypothetical protein | 2.00 | 2.30 | |
| SA0271 | Conserved hypothetical protein | 2.41 | 3.90 | |
| SA0275 | Conserved hypothetical protein | 2.18 | 2.16 | |
| SA0331 | Conserved hypothetical protein | 0.31 | 0.33 | |
| SA0332 | Conserved hypothetical protein | 0.23 | 0.35 | |
| SA0333 | Conserved hypothetical protein | 0.26 | 0.35 | |
| SA0394 | Hypothetical protein | 0.30 | 0.24 | |
| SA0663 | Hypothetical protein | 0.32 | 0.48 | |
| SA0737 | Hypothetical protein | 2.90 | 2.28 | |
| SA0738 | Hypothetical protein | 2.55 | 2.37 | |
| SA0739 | Conserved hypothetical protein | 2.86 | 5.11 | |
| SA0741 | Conserved hypothetical protein | 3.93 | 4.14 | |
| SA0742 | Hypothetical protein | 3.74 | 3.29 | |
| SA0761 | Conserved hypothetical protein | 2.54 | 6.06 | |
| SA0830 | Conserved hypothetical protein | 2.49 | 2.63 | |
| SA1002 | Hypothetical protein | 2.40 | 3.25 | |
| SA1049 | Hypothetical protein | 0.41 | 0.37 | |
| SA1222 | Hypothetical protein | 2.04 | 2.49 | |
| SA1268 | Hypothetical protein | 0.36 | 0.25 | |
| SA1664 | Conserved hypothetical protein | 2.22 | 2.23 | |
| SA1755 | Hypothetical protein (bacteriophage ϕN315) | 0.25 | 0.188 | |
| SA2299 | Conserved hypothetical protein | 2.70 | 2.04 | |
| SA2491 | Conserved hypothetical protein | 2.49 | 2.55 | |
Locus tags are based on S. aureus strain N315. ORF, open reading frame.
MET, methicillin; PTS, phosphotransferase system.