Fig 3.

Representative data from an initial antibacterial screen using S. aureus (ATCC 25923) and E. coli (ATCC 25922) showing the average percent inhibitions (a) and the percent inhibitions with thiourea (b), triazole (c), and urea (d) linkers. Cultures at an optical density of 0.005 at 595 nm were treated with compound at a final concentration of 5 μM in cation-adjusted Mueller-Hinton Broth II. Cultures were incubated for 20 h at 35°C. The optical density at 595 nm was recorded, and the percent inhibition was calculated. N, neomycin; H258, Hoechst 33258; H342. Hoechst 33342; A, ampicillin; P, penicillin; B, bacitracin.