Fig 2.
Effect of COBI and RTV on the transport of tenofovir by OAT1v1 (A), OAT1v2 (B), and OAT3 (C). Cells expressing OATs were incubated with 1.2 μM [3H]TFV and either DMSO or serially diluted tested compounds for 3 to 6 min (OAT1) or 10 min (OAT3) at 37°C. The intracellular level of [3H]TFV was quantified, and the percentage of OAT1- or OAT3-specific uptake relative to that for the untreated control was calculated after subtracting background determined in the presence of 50 μM probenecid. Results represent means ± SD from at least three independent experiments.